Why is rice vinegar not gluten-free?

Rice vinegar, also known as rice wine vinegar, is a staple condiment in many Asian cuisines. It is made from fermented rice and has a mild, slightly sweet flavor. Rice vinegar adds a bright, acidic note to dishes like sushi rice, salad dressings, marinades, and dipping sauces.

Many people assume that since rice vinegar is made from rice, it must be gluten-free. However, traditional rice vinegar is actually not gluten-free due to the way it is produced. The fermentation process involves barley or wheat.

So why exactly is rice vinegar not gluten-free? Here is a deeper look at how traditional rice vinegar is made and why it contains traces of gluten.

How Rice Vinegar Is Made

Rice vinegar is made by fermenting the sugars in rice into alcohol. This alcoholic rice wine is then further fermented to convert the alcohol into organic acids, resulting in an acidic liquid.

Here are the main steps to make rice vinegar:

1. Making Rice Wine

Rice is first cooked and combined with koji mold to kickstart the fermentation process. Koji is made by culturing steamed grains like rice or barley with a fungus called Aspergillus oryzae. This koji mold helps break down the starches in rice into glucose.

The glucose is fermented into rice wine with yeast. The main byproduct of this fermentation is ethanol alcohol. At this stage, the product is an alcoholic rice wine, similar to sake.

2. Vinegar Fermentation

To convert the rice wine into vinegar, another fermentation takes place. The ethanol is oxidized into acetic acid by acetic acid bacteria.

This bacterial fermentation step is where the gluten contamination can occur in rice vinegar production.

Why Rice Vinegar Contains Gluten

Here are the main reasons why rice vinegar often contains traces of gluten:

It’s Brewed with Barley or Wheat

In the initial koji fermentation stage, rice vinegar producers often use barley or wheat along with the rice. Barley and wheat contain gluten.

As the koji mold grows, it produces enzymes that break down proteins, carbohydrates and starches in both the rice and gluten grains.

This makes the resulting rice wine susceptible to gluten cross-contamination. The gluten proteins can then carry over into the final vinegar.

It’s Fermented with Wheat Bran

Some manufacturers add wheat bran to the fermentation tanks during the second vinegar fermentation step.

The wheat bran provides nutrients to help the acetic acid bacteria thrive and convert the alcohol into vinegar. It also helps absorb excess oxygen.

Since wheat bran contains gluten, adding it during fermentation can introduce gluten into the final rice vinegar.

It’s Filtered Through Wheat Chaff

After fermentation, the vinegar is filtered, pasteurized and bottled. Traditionally, the filtration process involves straining the vinegar through a cloth or filter made of wheat chaff.

Wheat chaff is the dry, scaly husk surrounding wheat grains. It contains traces of gluten, which can get filtered into the finished vinegar.

The Equipment is Not Thoroughly Cleaned

Rice vinegar facilities often also produce other gluten-containing vinegars, like malt vinegar made from barley.

If equipment isn’t thoroughly cleaned between batches, it can result in cross-contamination between the gluten and gluten-free products.

It’s Blended with Malt Vinegar

Some brands blend their rice vinegar with malt vinegar, which is made from barley. Barley malt vinegar contains gluten, so blending introduces gluten into the final product.

These are the key reasons why most traditionally brewed rice vinegars are not gluten-free. Small amounts of gluten can get introduced at various stages and are not fully removed.

Labeling Standards for Gluten-Free Rice Vinegar

In many countries, there are labeling regulations around using the term “gluten-free” on foods:

United States

– The FDA defines gluten-free as less than 20 parts per million (ppm) of gluten.

– Any product labeled gluten-free must meet this standard.


– Foods labeled gluten-free must have less than 20 ppm of gluten.

European Union

– Foods must contain less than 20 ppm of gluten to be labeled as gluten-free.

– They also cannot contain wheat, rye, barley, oats or their hybridized strains.


– The allowed gluten limit is “no detectable gluten.”

– There is no defined ppm level.

So if a rice vinegar is labeled as “gluten-free” in these countries, it has been tested to verify it meets the accepted standard for gluten content.

Testing Methods for Gluten in Rice Vinegar

There are several lab methods used to detect the presence of gluten in rice vinegar. Here are some of the main testing techniques:

ELISA Testing

The ELISA (enzyme-linked immunosorbent assay) test is the most common method. It uses antibodies that bind specifically to gluten proteins. If the antibodies detect gluten proteins in a vinegar sample above 20 ppm, the vinegar fails the gluten-free test.

Mass Spectrometry

This method identifies gluten peptide sequences by their mass. The vinegar sample is analyzed for peptides that match sequences present in gluten-containing grains.

PCR Testing

PCR (polymerase chain reaction) can detect the presence of DNA from gluten sources like wheat, rye or barley. A positive PCR test means the vinegar contains gluten from one of these grains.

Lateral Flow Devices

Lateral flow devices provide a rapid test strip to detect gluten. The vinegar sample migrates up the strip, and if gluten is present, it will bind to antibodies and generate a visible line on the strip.

These sensitive lab techniques allow rice vinegar producers to accurately test for gluten and verify the gluten-free status of their products. Rice vinegars that test below 20 ppm can be safely labeled gluten-free.

Types of Rice Vinegars

There are several types of rice vinegars made using different methods. Here is an overview of some common varieties:

Traditional Rice Vinegar

– Brewed from rice, with barley and wheat
– Contains gluten
– Examples: Marukan and Mizkan rice vinegars

Gluten-Free Rice Vinegar

– Made purely from rice, without gluten grains
– Tested to ensure no cross-contamination
– Examples: Eden Foods and O Organics rice vinegars

Black Rice Vinegar

– Made from black rice
– Usually gluten-free
– Higher antioxidant content
– Example: Gold Plum Chinkiang black rice vinegar

Brown Rice Vinegar

– Unpolished brown rice
– Richer flavor
– Usually gluten-free
– Example: Bluegrass Soy organic brown rice vinegar

Red Rice Vinegar

– Made from red yeast rice
– No gluten
– Example: Pagoda Chinese red rice vinegar

Sanbaizu Vinegar

– Blend of rice, wheat, and malt vinegars
– Contains gluten due to wheat
– Milder acidity
– Example: Mitoku Organic Sanbaizu vinegar

So when choosing a rice vinegar, opt for a certified gluten-free variety to avoid gluten exposure. Traditional rice vinegars are more likely to contain traces of gluten.

Rice Vinegars Labeled Gluten-Free

Here are some recommended brands of rice vinegar that clearly label their products as gluten-free:

Eden Foods Organic Rice Vinegar

– Made from organic whole grain brown rice
– Naturally brewed, no added sugar
– Non-GMO and gluten-free

Marukan Organic Rice Vinegar

– Unseasoned rice vinegar
– Lab tested gluten-free
– Certified organic and non-GMO

O Organics Unseasoned Rice Vinegar

– Made purely from rice
– USDA certified organic
– Verified gluten-free

Mizkan Rice Vinegar

– Produced using 100% rice
– Lab tested to be gluten-free
– Versatile for dressings, marinades, pickles

Bluegrass Soy Organic Brown Rice Vinegar

– Rich brown rice vinegar
– Non-GMO, gluten-free
– Smooth, mellow flavor

When a rice vinegar specifies “gluten-free” on the label, it assures it has been tested to verify the gluten level is below 20 ppm.

Is Brown Rice Vinegar Gluten-Free?

Brown rice vinegar is naturally gluten-free. Since it is made purely from brown rice with no gluten grains added, it does not contain gluten.

Brown rice is simply unpolished rice with the nutrient-dense bran layer left intact. The bran gives brown rice vinegar a rich, complex flavor compared to white rice vinegar.

Reputable brands of brown rice vinegar like Marukan, Eden Foods and O Organics label their products as gluten-free. They separately produce and bottle the brown rice vinegar to prevent cross-contamination with gluten.

So you can safely use brown rice vinegar on a gluten-free diet. The brown rice gives it more vitamins, minerals and antioxidants than refined white rice vinegar.

Is Black Rice Vinegar Gluten-Free?

Yes, black rice vinegar is gluten-free. It is made by fermenting black rice, which does not contain any gluten.

Black rice gets its dark purple hue from anthocyanins, beneficial antioxidants that give the vinegar extra health perks. The rich color is also ideal for making naturally-tinted pickles.

High quality brands clearly label their black rice vinegar products as gluten-free, like Gold Plum Chinkiang Vinegar. They take steps to avoid cross-contamination during the production process.

Black rice vinegars from China can be questionable in terms of gluten content and may list wheat as an ingredient. Opt for brands made in the U.S., Canada or the E.U. to guarantee gluten-free status.

Is Mirin Gluten-Free?

Traditional mirin is not gluten-free. Mirin is a sweetened rice wine used frequently in Japanese cuisine. It provides a touch of sweetness with less alcohol bite than sake or rice wine vinegar.

Authentic mirin brewed in Japan contains about 1-2% alcohol along with rice and koji. The koji is usually made from a mixture of rice, barley and/or wheat.

So traditional mirin that uses barley or wheat koji introduces gluten into the final product. It is also often distilled, which does not remove gluten.

However, you can find gluten-free mirin substitutes made using just rice koji or molasses to replace the sweetness of sugar. Look for “gluten-free” on the label when buying mirin.

Substitutes for Rice Vinegar

If you need a gluten-free swap for rice vinegar, here are some alternative vinegars to consider:

– Apple cider vinegar
– White wine vinegar
– Champagne vinegar
– Sherry vinegar
– Balsamic vinegar
– Lemon or lime juice
– Rice wine (sake)
– Gluten-free rice vinegar-based seasoning

Apple cider vinegar and white wine vinegar work well as replacements in dishes like sushi rice, sauces, dressings and pickling recipes. Start with a smaller amount than rice vinegar and adjust to taste.

You can also make your own blend with vinegar, sugar, salt and sake to mimic the profile of rice vinegar. Overall, look for vinegars that are naturally gluten-free and clearly labeled.

Is Rice Vinegar Safe on a Gluten-Free Diet?

Gluten-free rice vinegar that is specifically produced and tested to contain under 20ppm of gluten is generally safe for people on gluten-free diets.

However, those with celiac disease or severe gluten intolerances should use extra caution with rice vinegar and all vinegars.

Some individuals are highly sensitive and react to tiny amounts of gluten exposure. There is also a risk of trace amounts of gluten remaining even in “gluten-free” vinegars.

So consult your doctor or dietitian about your individual tolerance level. They may recommend avoiding or limiting vinegar intake to prevent accidental gluten ingestion.

Tips for Cooking and Baking with Rice Vinegar

Here are some useful tips for cooking and baking with rice vinegar:

– Substitute 1 tablespoon rice vinegar for 1 tablespoon of mild vinegar like cider vinegar or white wine vinegar in recipes

– Add a splash of rice vinegar to protein dishes, salad dressings, sauces and marinades for a punch of flavor

– Use an equal amount of rice vinegar in place of other vinegars when pickling fruits or vegetables

– Combine 2 tablespoons rice vinegar with 1 tablespoon sugar or honey for a quick rice vinegar sweetener

– Add a few drops of rice vinegar to give smoothies or desserts a tangy twist

– For sushi rice, combine 1/4 cup rice vinegar, 2 tablespoons sugar and 1 teaspoon salt. Mix into cooked rice while hot.

– In baked goods, substitute 1 teaspoon rice vinegar for 1 teaspoon lemon juice to help activation baking soda for leavening

Rice vinegar is multifunctional in both savory dishes and baked goods. Start with small amounts until you get a feel for its unique sweet-sour taste.


While rice vinegar has a gluten-free sounding name, traditional types often contain traces of gluten from the brewing process. Looking for “gluten-free” certification on the label ensures the product tests below 20ppm of gluten.

Opt for rice vinegars made purely from rice without other gluten-containing grains. Brown rice, black rice and red rice vinegars are good gluten-free choices.

Mirin, sanbaizu and seasoned rice vinegars typically contain gluten and must be avoided. Overall, vinegars are one ingredient that requires scrutiny to find guaranteed gluten-free varieties.

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